Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 84, Issue 8, Pages 1685-1688Publisher
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2020.1756737
Keywords
Cancer; lipophilic carbocyanine dye; lipid membrane; micronucleus; nuclear envelope
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Funding
- Novartis Foundation Japan for the Promotion of Science [JP17H01878]
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Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.
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