Journal
BIORESOURCE TECHNOLOGY
Volume 312, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123580
Keywords
Microbial lipid; Rice straw; Concentrated hydrolysis; Glycerol; Pretreatment
Funding
- National Natural Science Foundation of China [21878161]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_0605]
- Nanjing Agricultural University [68Q-0603]
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Microbial lipids were produced by both rice straw hydrolysates and recycled pretreated glycerol. First, lipid fermentation of glucose via Cryptococcus curvatus was optimized by response surface methodology. Variables were selected by Plackett-Burman design, and optimized by central composite design, achieving 4.9 g/L total lipid and 0.16 g/g lipid yield, and increased further as glucose increased from 30 to 50 g/L. Secondly, after pretreatment, 72% lignin of rice straw was removed with glucose yield increased by 2.4 times to 74% at 20% substrate and 3 FPU/g. Subsequently, its hydrolysates produced high total lipid (8.8 g/L) and lipid yield (0.17 g/g). Finally, recycled glycerol reached the maximum total lipid of 7.2 g/L and high lipid yield of 0.16 g/g. Based on the calculation, 2.9 g total lipid would be produced from 1 g rice straw and the recycled glycerol, with a similar composition to soybean oil.
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