Journal
BIORESOURCE TECHNOLOGY
Volume 303, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.122942
Keywords
Bioethanol; Air currents assisted particle separation (ACAPS); beta-Glucan; Barley; Starch hydrolysis; Ethanol fermentation; STARGEN 002; Biorefinery
Funding
- Future Energy Systems [T01-P01]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN2019-04184]
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Isolation of fiber concentrate enriched in beta-glucan from barley flour via air currents assisted particle separation (ACAPS) generates an underutilized by-product stream, starch concentrate. Since barley starch concentrate (BSC) is depleted in soluble fibre, we examined the enzyme requirements for its hydrolysis and subsequent fermentation. Lower doses of a common raw starch hydrolyzing enzyme (STARGEN (TM) 002) effectively hydrolyzed BSC, achieving similar hydrolysis kinetics to the wheat benchmark. Hydrolysis of BSC did not require further enzyme supplementation, which is required for optimal wheat hydrolysis. This likely resulted from the smaller particle size of BSC relative to wheat feedstocks. Interestingly, simultaneous saccharification and fermentation of BSC using a 0.25X dose of STARGEN (TM) 002 alone enabled efficient ethanol production, though a requirement for phosphorus supplementation was identified. This study proposes a biorefining strategy that supports the generation of a value-added co-product, starch concentrate, while significantly reducing the enzyme requirements for bioethanol production.
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