Anti-bacterial and anti-biofilm properties of green synthesized copper nanoparticles from Cardiospermum halicacabum leaf extract

In the present study, a copper nanoparticle (Cu NPs) was synthesized by a green synthesis method with Cardiospermum halicacabum leaf extract. The surface area of Cu NPs was measured with dynamic light scattering (DLS). UV-Vis spectrum clearly illustrates the typical absorption peak of Cu NPs. The crystalline property of Cu NPs was confirmed from the XRD pattern. TEM analysis clearly indicates the average particle size of synthesized Cu NPs was in the range of 30-40 nm with hexagonal shape. Energy-dispersive spectroscopy confirms the major strong peaks of Cu NPs. FTIR analysis confirms the existence of various functional biomolecules over the metal nanoparticles and they are playing an important role in the formation of Cu NPs. The antibacterial and anti-biofilm analyses were carried out to confirm their aptitude for biomedical applications. Interestingly, Cu NPs control the development of biofilm by attaching over the cell wall and disturb their growth and development.