m7GHub: deciphering the location, regulation and pathogenesis of internal mRNA N7-methylguanosine (m7G) sites in human

Motivation: Recent progress in N7-methylguanosine (m7G) RNA methylation studies has focused on its internal (rather than capped) presence within mRNAs. Tens of thousands of internal mRNA m(7)G sites have been identified within mammalian transcriptomes, and a single resource to best share, annotate and analyze the massive m(7)G data generated recently are sorely needed. Results: We report here m(7)GHub, a comprehensive online platform for deciphering the location, regulation and pathogenesis of internal mRNA m(7)G. The m(7)GHub consists of four main components, including: the first internal mRNA m(7) G database containing 44 058 experimentally validated internal mRNA m(7) G sites, a sequence-based high-accuracy predictor, the first web server for assessing the impact of mutations on m7 G status, and the first database recording 1218 disease-associated genetic mutations that may function through regulation of m(7)G methylation. Together, m(7)GHub will serve as a useful resource for research on internal mRNA m(7)G modification.