Journal
CATALYSIS TODAY
Volume 259, Issue -, Pages 197-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cattod.2015.06.032
Keywords
Nucleoside 2 '-deoxyribosyltransfe rase; Biotransformations; Enzyme immobilization; Nucleoside analogues; Decytabine; Trifluridine
Funding
- Comunidad Autonoma de Madrid (Spain) [52009/PPQ-1752]
- Spanish Ministry of Science and Innovation [CTQ2009-11543]
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The immobilization of Bacillus psychrosaccharolyticus nucleoside 2'-deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis of trifluridine and decytabine obtaining conversions ranging from 50% to 76%. (C) 2015 Elsevier B.V. All rights reserved.
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