Seasonal changes and the influence of tree species and ambient temperature on the fission-fusion dynamics of tree-roosting bats

Many species of bats live in dynamic associations in which individuals aggregate or split into smaller or larger groups, moving between roost sites, but factors influencing group size and roost switching decisions in bats living in fission-fusion societies are poorly understood. To test the extent to which fission-fusion dynamics are species-specific and shaped by environmental factors, we radio-tracked two closely related species: noctule bats Nyctalus noctula and Leisler's bats N. leisleri in well-preserved, old-growth stands of the Biaowiea Forest, where roost availability and diversity is high and bats can potentially freely exhibit fission-fusion behaviour. We estimated the number of bats per roost and noted the tree species selected as roost sites. We evaluated the influence of ambient temperature and roost tree species on bat group size and the process of roost switching, and their seasonal component. Our results revealed that bat group size showed species-specific differences in seasonal variation, relationship to ambient temperature and tree species. The number of noctule bats emerging from a roost was relatively stable with a weak tendency to decrease during the breeding season while the number of Leisler's bats emerging from a roost increased when the juveniles started to be volant. Group size of noctule bats increased with ambient temperature. Leisler's bats formed smaller colonies in oaks and ashes than in other tree species. Roost switching rate was associated with ambient temperature and tree species selected by the bats. The bats changed roosts more frequently when the minimum ambient temperature increased, and less often in ashes than in oaks and other tree species. Our results show that bat group size and roost switching behaviour are shaped by both environmental factors and the stage of reproduction.Significance statementEnvironmental factors may affect group size and roost switching behaviour of bats forming fission-fusion associations, but this is expected to vary depending on body size, environmental conditions, as well as seasonally. Few studies have explored this behaviour in tree-dwelling bats and examined how it differs between species. Here, we studied the group size dynamics and the process of roost switching in closely related bat species living in well-preserved, European old-growth forests, for which the knowledge of these patterns is particularly poor. We demonstrate for the first time that bat group size and roost switching rate relates to roost tree species selected by bats, the ambient temperature and varies seasonally, and that these effects are species-specific. The results point out the importance of the availability of suitable roost trees providing a range of microclimatic conditions for maintaining efficient fission-fusion dynamics throughout the reproductive period.