Journal
ARCHIVES OF MICROBIOLOGY
Volume 202, Issue 7, Pages 1729-1739Publisher
SPRINGER
DOI: 10.1007/s00203-020-01884-1
Keywords
Yeast; Hydrolysis; beta-Glucosidase; Cellobiase; Fermentation; Atlantic rain forest
Categories
Funding
- National Council for Scientific and Technological Development (CNPq) [454215/2014-2, 308627/2015-6, 308389/2019-0]
- Research and Innovation Support Foundation of Santa Catarina State (FAPESC) [749/2016, T.O. 2016TR2188]
- Support Program for Scientific and Technological Initiation from Federal University of Fronteira Sul (PRO-ICT/UFFS)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
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We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular beta-glucosidase activity [similar to 200 U (g cells)(-1) for 200 mM cellobiose and similar to 100 U (g cells)(-1) for 2 mM pNP beta G] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L-1 of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with K-m of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular beta-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.
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