Journal
AQUACULTURE RESEARCH
Volume 51, Issue 6, Pages 2573-2581Publisher
WILEY
DOI: 10.1111/are.14599
Keywords
Bursicon; environmental stress; haemocyte granulation; immune gene response; Macrobrachium rosenbergii; neurohormone
Categories
Funding
- Centex Shrimp, Faculty of Science, Mahidol University [3962-11814]
- Mahidol University [3962-11814]
Ask authors/readers for more resources
We examined the effects of salinity stress and ammonia stress on alteration of the haemocyte count, phenoloxidase activity, expressions of immune-related genes including prophenoloxidase (proPO), crustin, heat shock protein 70 (HSP70), and expressions of stress-responsive neurohormone (Bur-alpha and Bur-beta) in the thoracic and abdominal ganglia of giant river prawn Macrobrachium. These parameters of prawn that subjected to salinity stress (transferred from 0 parts per thousand to 5 parts per thousand and 10 parts per thousand), and subjected to ammonia-nitrogen (ammonia-N) stress (transferred from 0 to 0.262 mg/L and 0.786 mg/L) were examined after 0, 3, 6, 24, 72 and 168 hr respectively. During the initial period of 3, 6 and 24 hr, granulocyte haemocyte (granular and semi-granular hemocyte) count and PO activity significantly decreased, while expressions of Bur-alpha and Bur-beta significantly increased. After 24 hr, granulocyte haemocyte count and PO activity significantly increased, whereas expressions of Bur-alpha and Bur-beta significantly decreased. The expressions of proPO, crustin and HSP70 were significantly downregulated in the prawn that subjected to salinity stress and ammonia-N stress at all time periods of 3-168 hr. In conclusion, changes in the granulocyte haemocyte count of M. rosenbergii following salinity stress and ammonia-N stress are closely associated with the changes of Bur-alpha and Bur-beta expressions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available