4.4 Article

Ionic Liquid Tolerance of Yeasts in Family Dipodascaceae and Genus Wickerhamomyces

Journal

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 191, Issue 4, Pages 1580-1593

Publisher

SPRINGER
DOI: 10.1007/s12010-020-03293-y

Keywords

Ionic liquid pretreatment; Lignocellulosic biofuels; Inhibitor tolerance; Switchgrass hydrolysate; Tolerant yeast; Galactomyces candidus

Funding

  1. Office of Science, Office of Biological and Environmental Research, of the US Department of Energy [DE-AC02-05CH11231]

Ask authors/readers for more resources

In previous studies of ionic liquid (IL) tolerance of numerous species of ascomycetous yeasts, two strains of Wickerhamomyces ciferrii and Galactomyces candidus had unusually high tolerance in media containing up to 5% (w/v) of the 1-ethyl-3-methylimidazolium acetate ([C(2)C(1)Im][OAc]). The study aimed at investigating whether additional strains of these species, and additional species in the Dipodascaceae family, also possess IL tolerance, and to compare sensitivity to the acetate and chloride versions of the ionic liquid. Fifty five yeast strains in the family Dipodascaceae, which encompasses genera Galactomyces, Geotrichum, and Dipodascus, and seven yeast strains of species Wickerhamomyces ciferrii were tested for ability to grow in laboratory medium containing no IL, 242 mM [C(2)C(1)Im][OAc], or 242 mM [C(2)C(1)Im]Cl, and in IL-pretreated switchgrass hydrolysate. Many yeasts exhibited tolerance of one or both ILs, with higher tolerance of the chloride anion than of the acetate anion. Different strains of the same species exhibited varying degrees of IL tolerance. Galactomyces candidus, UCDFSTs 52-260, and 50-64, had exceptionally robust growth in [C(2)C(1)Im][OAc], and also grew well in the switchgrass hydrolysate. Identification of IL tolerant and IL resistant yeast strains will facilitate studies of the mechanism of IL tolerance, which could include superior efflux, metabolism or exclusion.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available