Journal
ANTICANCER RESEARCH
Volume 40, Issue 4, Pages 1843-1853Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.14138
Keywords
Epithelial-mesenchymal transition; pancreatic cancer; exosomes; micro RNA
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Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [16K10590]
- Grants-in-Aid for Scientific Research [16K10590] Funding Source: KAKEN
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Background/Aim: Epithelial-to-mesenchymal transition (EMT) plays important roles in cancer progression. This study aimed to identify the exosomal miRNA (exo-miRNA) profiles related to the EMT status in pancreatic cancer (PC). Materials and Methods: Comprehensive exo-miRNA-expression profiles in the culture media of PC cell lines were analyzed through microarray technology. The identified miRNAs were analyzed to investigate their clinical implication using The Cancer Genome Atlas (TCGA) dataset and clinical samples. Results: We prioritized 291 exo-miRNAs differentially expressed between epithelial and mesenchymal cell types. Among them, survival analysis based on the TCGA dataset revealed that mir-196b and mir-204 significantly stratify the prognosis of PC cases. In addition, analysis of cell lines indicated miR-196b-3p as a mesenchymal marker and miR-204-3p as an epithelial marker. Finally, we demonstrated that miR-196b-3p and miR-204-3p in serum exosomes were differentially expressed among intraductal papillary mucinous neoplasms, mucinous cystic neoplasms, and PC. Conclusion: Serum exo-miRNA biomarkers potentially identify the pancreatic tumor status through less-invasive methods.
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