Journal
ANTICANCER RESEARCH
Volume 40, Issue 5, Pages 2675-2685Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.14238
Keywords
N-farnesyl-norcantharimide; Jurkat T cells; next-generation sequencing; tumor suppressor gene; biosynthesis
Categories
Funding
- Taipei Veterans General Hospital, Taipei, Taiwan [V104C-153, V104E5001]
Ask authors/readers for more resources
Background/Aim: To evaluate the anti-cancer mechanism of N-Farnesyl-norcantharimide (NC15). Materials and Methods: The viability of NC15-treated human leukemic Jurkat T (JKT) cells was assessed using the Kit-8 cell counting method. Flow cytometry analysis, human apoptosis antibody array assay, and whole genome sequencing were adopted to investigate the mechanism underlying the anti-cancer activity of NC15 in JKT cells. Results: The growth inhibition rates of NC15 in JKT cells were about 80% and 95% after treatment with 8 mu mol/l NC15 for 24 and 48 h, respectively. The percentages of NC15-treated JKT cells in the sub-G(1) phase at 24 and 48 h were 22.0% and 34.3%, respectively, in contrast to the 1.5% in the control. Next-generation sequencing showed that many tumor suppressor genes (TSG) were up-regulated, while many genes associated with steroid biosynthesis, metabolic pathways, and fatty acid metabolism were down-regulated. Conclusion: NC15 can reduce the cell viability and increase the percentage of JKT cells in the sub-G(1) phase by up-regulating TSG and related genes, and down-regulating the genes for steroid biosynthesis, metabolic pathways and fatty acid metabolism, instead of through apoptosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available