Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 37, Pages 15987-15991Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202006964
Keywords
bioimaging; bioinorganic chemistry; inorganic photochemistry; iridium; three-photon excitation
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Funding
- National Science Foundation of China [21525105, 21778079, 21701196, 21807119]
- Ministry of Education of China [IRT-17R111]
- Fundamental Research Funds for the Central Universities [20lgjc01]
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Compared to 2PE (two-photon excitation) microscopy, 3PE microscopy has superior spatial resolution, deeper tissue penetration, and less defocused interference. The design of suitable agents with a large Stokes shift, good three-photon absorption (3PA), subcellular targeting, and fluorescence lifetime imaging (FLIM) properties, is challenging. Now, two Ir(III)complexes (3PAIr1 and 3PAIr2) were developed as efficient three-photon phosphorescence (3PP) agents. Calculations reveal that the introduction of a new group to the molecular scaffold confers a quadruple promotion in three-photon transition probability. Confocal and lifetime imaging of mitochondria using Ir(III)complexes as 3PP agents is shown. The complexes exhibit low working concentration (50 nm), fast uptake (5 min), and low threshold for three-photon excitation power (0.5 mW at 980 nm). The impressive tissue penetration depth (ca. 450 mu m) allowed the 3D imaging and reconstruction of brain vasculature from a living specimen.
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