Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 24, Pages 9282-9287Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202004326
Keywords
elastomer; hydrogen peroxide; imaging; liquid crystal; single cell
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Funding
- National Natural Science Foundation of China [21804026, 21727814, 21621003]
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Live-imaging of signaling molecules released from living cells is a fundamental challenge in life sciences. Herein, we synthesized liquid crystal elastomer microspheres functionalized with horse-radish peroxidase (LCEM-HRP), which can be immobilized directly on the cell membrane to monitor real-time release of H2O2 at the single-cell level. LCEM-HRP could report H2O2 through a concentric-to-radial (C-R) transfiguration, which is due to the deprotonation of LCEM-HRP and the break of inter or intra-chain hydrogen bonding in LCEM-HRP caused by HRP-catalyzed reduction of H2O2. The level of transfiguration of LCEM-HRP revealed the different amounts of H2O2 released from cells. The estimated detection sensitivity was approximate to 2.2x10(-7) mu m for 10 min of detection time. The cell lines and cell-cell heterogeneity was explored from different configurations. LCEM-HRP presents a new approach for in situ real-time imaging of H2O2 release from living cells and can be the basis for seeking more advanced chemical probes for imaging of various signaling molecules in the cellular microenvironment.
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