Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 26, Pages 10327-10331Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201916718
Keywords
aggregation-induced emission; centromeres; chromosome analysis; chromosome periphery; fluorescence imaging
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Funding
- Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet
- National Natural Science Foundation of China [21708030]
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The chromosome periphery (CP) is a complex network that covers the outer surface of chromosomes. It acts as a carrier of nucleolar components, helps maintain chromosome structure, and plays an important role in mitosis. Current methods for fluorescence imaging of CP largely rely on immunostaining. We herein report a small-molecule fluorescent probe,ID-IQ, which possesses aggregation-induced emission (AIE) property, for CP imaging. By labelling the CP,ID-IQsharply highlighted the chromosome boundaries, which enabled rapid segmentation of touching and overlapping chromosomes, direct identification of the centromere, and clear visualization of chromosome morphology.ID-IQstaining was also compatible with fluorescence in situ hybridization and could assist the precise location of the gene in designated chromosome. Altogether, this study provides a versatile cytogenetic tool for improved chromosome analysis, which greatly benefits the clinical diagnostic testing and genomic research.
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