Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 24, Pages 9719-9726Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202002422
Keywords
fluorescence lifetime imaging microscopy; fluorescent probes; G-quadruplexes; photon counts; structure elucidation
Categories
Funding
- National Science Foundation of China [21837006, 91953117, 21572282]
- Ministry of Education of China [IRT-17R111]
- Science and Technology Planning Project of Guangdong Province [2013B051000047, 2020A1515011439, 207999]
- Fundamental Research Funds for the Central Universities
Ask authors/readers for more resources
G-quadruplex DNA show structural polymorphism, leading to challenges in the use of selective recognition probes for the accurate detection of G-quadruplexes in vivo. Herein, we present a tripodal cationic fluorescent probe, NBTE, which showed distinguishable fluorescence lifetime responses between G-quadruplexes and other DNA topologies, and fluorescence quantum yield (phi(f)) enhancement upon G-quadruplex binding. We determined two NBTE-G-quadruplex complex structures with high phi(f) values by NMR spectroscopy. The structures indicated NBTE interacted with G-quadruplexes using three arms through pi-pi stacking, differing from that with duplex DNA using two arms, which rationalized the higher phi(f) values and lifetime response of NBTE upon G-quadruplex binding. Based on photon counts of FLIM, we detected the percentage of G-quadruplex DNA in live cells with NBTE and found G-quadruplex DNA content in cancer cells is 4-fold that in normal cells, suggesting the potential applications of this probe in cancer cell detection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available