Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 10, Pages 7139-7145Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c00570
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Funding
- Ministry of Science and Technology (MOST), R.O.C. [109-2636-M-002-005-]
- Center for Emerging Materials and Advanced Devices, National Taiwan University (NTU) [NTU-ERP-108L880116]
- NTU Consortia of Key Technologies
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A series of novel anthranilic acid derivatives I-IV, of which COOH-NH2 (I) and COOH-NHMe (IV) are endowed with acid and base bifunctionality, were designed and synthesized for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applications in dual polarity molecular imaging of biological samples, particularly for lipids. The heat of protonation, deprotonation, and proton transfer reaction as well as the capability of analyzing biomolecules in both positive and negative ion modes for I-IV were systematically investigated under standard 355 nm laser excitation. The results indicate correlation between dual polarity and acid-base property. Further, COOH-NHMe (IV) showed a unique performance and was successfully applied as the matrix for MALDI-TOP mass spectrometry imaging (MSI) for studying the mouse brain. Our results demonstrate superiority of COOH-NHMe (IV) in detecting more lipid and protein species compared to commercially available matrices. Moreover, MALDI-TOF MSI results were obtained for lipid distributions, making COOH-NHMe (IV) a potential next generation universal matrix.
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