Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 11, Pages 7581-7587Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c00125
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Funding
- National Natural Science Foundation of China [21874083, 21427808]
- Fundamental Research Funds of Shandong University [2018JC017]
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The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of similar to 835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and alpha-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg.mL(-1) to 0.1 ng.mL(-1) with a limit of detection of 1 fg mL(-1) (S/N = 3) as well as desired selectivity.
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