Journal
ANALYTICA CHIMICA ACTA
Volume 1104, Issue -, Pages 78-86Publisher
ELSEVIER
DOI: 10.1016/j.aca.2019.12.075
Keywords
Flower-type; Porous mesoporous silica; Chondroitin sulfate; Selective isolation; Low density lipoprotein
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Funding
- Natural Science Foundation of China [21974018, 21727811, 21575020]
- Fundamental Research Funds for the Central Universities [N170505002, N170504017, N170507001]
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Novel flower-type and three-dimensional porous nanoparticles are prepared for the isolation of low density lipoprotein (LDL). The amino-terminated dendritic mesoporous silica nanoparticles (A-DMSNs) show highly accessible central-radial pore (0.655 cm(3) g(-1)) and surface area (362.263 m(2) g(-1)), which play an important role in the superior adsorption capacity of 816.7 mu g mg(-1). The A-DMSNs is anchored with chondroitin sulfate (CS), shortly termed as ADC, for ensuring the selectivity of the adsorption. The adsorbed LDL is thereafter readily recovered at pH 9.0 by using 4 mmol L-1 Britton-Robinson buffer as stripping reagent, providing a recovery of 79.1%. ADC nanoparticles are further applied as sorbent for the selective isolation of LDL from simulated serum of hypercholesterolemia patient. High-purity LDL is achieved as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assays. (C) 2020 Elsevier B.V. All rights reserved.
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