Journal
ACS CHEMICAL BIOLOGY
Volume 15, Issue 4, Pages 1104-1114Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.0c00139
Keywords
-
Categories
Funding
- National Cancer Institute, National Institutes of Health [HHSN261200800001E]
- National Cancer Institute
- Intramural Research Program of the National Cancer Institute
- NATIONAL CANCER INSTITUTE [ZICBC011515, ZIABC011568, ZIABC011854] Funding Source: NIH RePORTER
Ask authors/readers for more resources
An automated, high-capacity, and high-throughput procedure for the rapid isolation and identification of biologically active natural products from a prefractionated library is presented. The semipreparative HPLC method uses 1 mg of the primary hit fraction and produces 22 subfractions in an assay-ready format. Following screening, all active fractions are analyzed by NMR, LCMS, and FTIR, and the active principle structural classes are elucidated. In the proof-of-concept study, we show the processes involved in generating the subfractions, the throughput of the structural elucidation work, as well as the ability to rapidly isolate and identify new and biologically active natural products. Overall, the rapid second-stage purification conserves extract mass, requires much less chemist time, and introduces knowledge of structure early in the isolation workflow.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available