Genetic Engineering-Facilitated Coassembly of Synthetic Bacterial Cells and Magnetic Nanoparticles for Efficient Heavy Metal Removal

Heavy-metal pollution is becoming a worldwide problem severely threatening our health and ecosystem. In this study, we constructed a genetic-engineering-driven coassembly of synthetic bacterial cells and magnetic nanoparticles (MNPs) for capturing heavy metals. The Escherichia coli cells were genetically engineered by introducing a de novo synthetic heavy-metal-capturing gene (encoding a protein SynHMB containing a six-histidine tag, two cystine-rich peptides, and a metallothionein sequence) and a synthetic type VI secretory system (T6SS) cluster of Pseudomonas putida, endowing the synthetic cells (SynEc2) with high ability of displaying the heavy-metal-capturing SynHMB on cell surface. MNPs were synthesized by a coprecipitation method and further modified by polyethylenimine (PEI) and diethylenetriaminepentaacetic acid (DTPA). Owing to the surface exposure of six-histidine tag on the synthetic bacteria and carboxyl groups on the modified MNPs (MNP@SiO2-PEI-DTPA), the synthetic bacterial cells and MNPs coassembled to form biotic/abiotic complex exhibiting a self-developing characteristic. In the culture medium containing both Cd2+ and Pb2+, the coassemblies captured these heavy metals with high removal efficiency (>90% even at 50 mg/L of Cd2+ and 50 mg/L of Pb2+) and were conveniently recycled by artificial magnetic fields. Moreover, the coassemblies realized coremoval of organic carbon pollutants with the removal efficiency of >80%. This study builds a novel biotic/abiotic coassembling platform facilitated by genetic engineering and sheds light on development of artificial magnetic biological systems for efficient treatment of environmental pollution.