Journal
ACS OMEGA
Volume 5, Issue 3, Pages 1414-1421Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.9b02919
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Funding
- High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CITTCD20170324]
- National Program for Special Support of Eminent Professionals
- Key Project at a central government level: The ability establishment of sustainable use for valuable Chinese medicine resources [2060302]
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Flavonoid glycosides have shown many pharmacological activities in clinical studies. However, the main way to obtain flavonoid glycosides is to extract and separate them from plants, which wastes both time and resources. Here, we identified the O-glycosyltransferase (UGTs) TwUGT3 from Tripterygium wilfordii and analyzed its bioinformatics. First, the enzyme was found to utilize phloretin and uridine diphosphate glucose (UDPG) as substrates to produce an acid-tolerant glucoside. Then, it also can use quercetin and UDPG as substrates to produce the corresponding O-glucoside. In addition, we further explored the substrate specificity of TwUGT3, which suggested that it also accepts luteolin, pinoccmbrin, and genistein to produce the corresponding O-glucosides. Subsequently, the optimum pH, reaction time, reaction temperature, and enzymatic kinetic parameters of TwUGT3 were determined.
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