Journal
PLANTS-BASEL
Volume 9, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/plants9030326
Keywords
digital PCR; pepino mosaic virus; quantification; genotype specific
Categories
Funding
- Euphresco Project VirusCollectII - Slovenian Ministry of Agriculture, Forestry and Food through the Administration of the Republic of Slovenia for Food Safety, Veterinary and Plant Protection [2015-F-132]
- Slovenian Research Agency [P4-0165, P4-0407]
- Metrology Institute of the Republic of Slovenia (MIRS)
- European Regional Development Fund
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In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide. At present, the main control strategy for prevention of PepMV disease in tomato production remains based on strict hygiene measures. To prevent damage caused by PepMV, cross-protection is used in some countries. Reliable characterisation, detection and quantification of the pathogen are vital for disease control. At present, reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) is generally used for this purpose. However, quantitative use of RT-qPCR is linked to standardised reference materials, which are not available for PepMV. In addition, many factors can influence RT-qPCR efficiencies and lead to lower accuracy of the quantification. In this study, well-characterised PepMV-genotype-specific RT-qPCR assays were transferred to two digital PCR (dPCR) platforms. dPCR-based assays allow absolute quantification without the need for standard curves, and due to the binary nature of the reaction, dPCR also overcomes many of the other drawbacks of RT-qPCR. We have shown that these newly developed and validated PepMV-genotype-specific dPCR assays are suitable candidates for higher-order methods for quantification of PepMV RNA, as they show lower measurement variability, with sensitivity and specificity comparable to RT-qPCR.
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