Journal
FOODS
Volume 9, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/foods9030281
Keywords
zearalenone; immunochromatographic assay; colloidal Au sphere; Au nanorods; cereals
Categories
Funding
- National Natural Science Foundation of China [31972147]
- Tianjin Technical Expert Project [19JCTPJC52700]
- Project of Tianjin Science and Technology Plan [18ZYPTJC00020]
- Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology [SKLFNS-KF-201907]
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This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 mu g L-1 and 60 mu g kg(-1), and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 mu g L-1 and 40 mu g kg(-1), respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B-1, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B-1. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.
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