Journal
FOODS
Volume 9, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/foods9010006
Keywords
front-face fluorescence; milk; riboflavin; prediction; rapid quantification
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Funding
- Spanish Ministerio de Economia y Competitividad [AGL2012-33957]
- European Regional Development Fund (ERDF/FEDER)
- National Scholarship Programme Programa Nacional de Becas y Credito Educativo (PRONABEC) from the Peruvian Government [019-2013-MINEDU-VMGI-OBEC-PRONABEC-OBPOST]
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The front-face fluorescence spectroscopy technique was used to establish a rapid prediction model of riboflavin concentration in milk without prior sample preparation. The prediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R-2 of 0.99 with a standard error of prediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk.
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