Journal
CARBON
Volume 97, Issue -, Pages 126-133Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.carbon.2015.08.051
Keywords
MWNTs; Gadolinium; HUVEC; Cell labelling; T-1-weighted MRI; Endosomal entrapment
Funding
- United Kingdom Engineering and Physical Sciences Research Council (EPSRC) [EP/G061882/1]
- European Commission FP-7 Marie Sklodowska Curie Actions-Career Development Research Fellowship (NANONEUROHOP) [PIEF-GA-2010-276051]
- EPSRC [EP/K005014/1, EP/G061882/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/G061882/1, EP/K005014/1] Funding Source: researchfish
Ask authors/readers for more resources
The development of efficient contrast agents for cell labelling coupled with powerful medical imaging techniques is of great interest for monitoring cell trafficking with potential clinical applications such as organ repair and regenerative medicine. In this paper, functionalised multi-walled carbon nanotubes (MWNTs) were engineered for cell labelling in T-1-weighted MRI applications. These sophisticated constructs were covalently functionalised with the gadolinium (Gd) chelating agent, diethylene triamine pentaacetic acid (DTPA), enabling tight attachment of Gd atoms onto the nanotube surface. The resulting Gd-labelled MWNTs were found to be stable over 2 weeks in water and mouse serum and high payload of Gd atoms could be loaded onto the nanotubes. The r(1) relaxivity of the Gd-MWNTs was a 3-fold higher than of the clinically approved T-1 contrast agent Magnevist at a magnetic field strength of 7T. The contrast efficiency, expressed as the r(1) relaxivity, of the Gd-MWNTs in Human Umbilical Vein Endothelial cells (HUVEC) was investigated at 7T and was found to be around 6.6 mM(-1) s(-1). There was no reduction of the contrast efficiency after internalisation in HUVECs, which was imparted to the ability of carbon nanotubes to translocate the cell membrane. (C) 2015 The Authors. Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available