Journal
CARBOHYDRATE RESEARCH
Volume 429, Issue -, Pages 23-28Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2016.04.023
Keywords
Ligand mimetics; STD NMR; PFG-STE NMR experiments; P. aeruginosa; Lectins; Dendrons
Funding
- PRIN [2011-2010L9SH3K]
- European Community's program NABBA [642028-H2020-MSCA-ITN-2014]
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Molecular recognition of glycans plays an important role in glycomic and glycobiology studies. For example, pathogens have a number of different types of lectin for targeting host sugars. In bacteria, lectins exist sometimes as domains of bacterial toxins and exploit adhesion to glycoconjugates as a means of entering host cells. Herein, we describe the synthesis of three glycodendrons with the aim to dissect the fine structural details involved in the multivalent carbohydrate-protein interactions. LecA, from the pathogen Pseudomonas aeruginosa, has been used to characterize galactose dendrons interaction using one of the most widespread NMR technique for the elucidation of receptor-ligand binding in solution, the saturation transfer difference (STD) NMR. Furthermore, the effective hydrodynamic radius of each dendrimer recognized by LecA was estimated from the diffusion coefficients determined by pulsed-field-gradient stimulated echo (PFG-STE) NMR experiments. (C) 2016 Elsevier Ltd. All rights reserved.
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