Journal
MICROORGANISMS
Volume 8, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms8010112
Keywords
Lactobacillus; Bifidobacterium; probiotic; simulated digestion; dose-response
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The probiotic definition stipulates adequate amounts. Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 x 10(9) colony forming units (CFU) and 7 x 10(10) CFU, of a probiotic mixture (Bifidobacterium lactis Bl-04, Lactobacillus acidophilus La-14, Lactobacillus paracasei Lpc-37, and Lactobacillus plantarum Lp-115) or placebo were tested. The four strains were quantified by qPCR and the metabolites analyzed by gas chromatography. There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for L. paracasei Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain. Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA). Higher doses of a species are required to reach measurable increases above the baseline level of this species.
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