Journal
ANTIOXIDANTS
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/antiox8120616
Keywords
dihydroethidium; lucigenin; luminol; tetrazolium salts; NADPH oxidase; cytochrome reductases
Funding
- National Health and Medical Research Council [1182948]
- National Health and Medical Research Council of Australia [1182948] Funding Source: NHMRC
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Reactive oxygen species (ROS) can be generated in mammalian cells via both enzymatic and non-enzymatic mechanisms. In sperm cells, while ROS may function as signalling molecules for some physiological pathways, the oxidative stress arising from the ubiquitous production of these compounds has been implicated in the pathogenesis of male infertility. In vitro studies have undoubtedly shown that spermatozoa are indeed susceptible to free radicals. However, many reports correlating ROS with sperm function impairment are based on an oxidative stress scenario created in vitro, lacking a more concrete observation of the real capacity of sperm in the production of ROS. Furthermore, sample contamination by leukocytes and the drawbacks of many dyes and techniques used to measure ROS also greatly impact the reliability of most studies in this field. Therefore, in addition to a careful scrutiny of the data already available, many aspects of the relationship between ROS and sperm physiopathology are still in need of further controlled and solid experiments before any definitive conclusions are drawn.
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