Journal
CARBOHYDRATE POLYMERS
Volume 146, Issue -, Pages 292-300Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.03.059
Keywords
Dendrobium huoshanense; Polysaccharide; Macrophage; Toll-like receptor 4; Signaling pathway
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Funding
- National Natural Science Foundation of China [20872024, 21006019, 31271814]
- project for Science and Technology Research Plan from Anhui Province of China [15czz04087]
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The present work aimed at investigating the pattern recognition receptor (PRR) and immunostimulatory mechanism of a purified Dendrobium huoshanense polysaccharide (DHP). We found that DHP could bind to the surface of macrophages and stimulate macrophages to secrete NO, TNF-alpha and IL-1 beta. To unravel the mechanism for the binding of DHP to macrophages, flow cytometry, confocal laser-scanning microscopy, affinity electrophoresis, SDS-PAGE and western blotting were employed to verify the type of PRR responsible for the recognition of DHP by RAW264.7 macrophages and peritoneal macrophages of C3H/HeN and C3H/HeJ macrophages. Results showed that toll-like receptor 4 (TLR4) was an essential receptor for macrophages to directly bind DHP. Further, the phosphorylation of ERK, JNK, Akt and p38 were observed to be time-dependently promoted by DHP, as well as the nuclear translocation of NF-kappa B p65. These results suggest that DHP activates macrophages via its direct binding to TLR4 to trigger TLR4 signaling pathways. (C) 2016 Elsevier Ltd. All rights reserved.
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