Journal
VACCINES
Volume 8, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/vaccines8010019
Keywords
FMDV; peptide vaccine; single dose; amount; pig
Categories
Funding
- Spanish Ministry of Science, Innovation and Universities [AGL2017-89097-C2, AGL2016-76445-R]
- ECFEDER funds [S2013/ABI-2906-PLATESA, P2018/BAA-4370]
- Generalitat de Catalunya [2009SGR492]
- Fundacion Ramon Areces
- Maria de Maeztu Program of the Spanish Ministry of Science, Innovation and Universities
- Spanish Ministry of Science, Innovation and University (FPI programme)
- Comunidad de Madrid
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Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals whose control relies on efficient vaccination. We have reported that dendrimer peptide B2T, with two copies of FMDV B-cell epitope VP1 (136-154) linked through maleimide units to T-cell epitope 3A (21-35)], elicits potent B- and T-cell specific responses and confers solid protection in pigs to type-O FMDV challenge after two doses of peptide. Herein we now show that B2T evokes specific protective immune responses after administration of a single dose of either 2 or 0.5 mg of peptide. High titers of ELISA and neutralizing antibodies against FMDV were detectable at day 15 post-immunization. Likewise, activated T cells and induced IFN-gamma response to in vitro recall with FMDV peptides were also detected by the same day. Further, in 70% of B2T-vaccinated pigs, full protection-no clinical signs of disease-was observed upon virus challenge at day 25 post-immunization. These results strengthen the potential of B2T as a safe, cost-effective candidate vaccine conferring adequate protection against FMDV with a single dose. The finding is particularly relevant to emergency scenarios permitting only a single shot immunization.
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