Journal
CARBOHYDRATE POLYMERS
Volume 139, Issue -, Pages 61-66Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.12.001
Keywords
Mung bean; Water-extractable polysaccharide; Ultrasonic
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Funding
- Hong Kong, Macao and Taiwan Science & Technology Cooperation Program of China [2014DFT30280, 2013DFH30050]
- special fund for Agro-scientific Research in the Public Interest [201403063]
- earmarked fund for China Agriculture Research System Modern Agro-industry Technology [CYTX-014]
- Agricultural Science and Technology Program for Innovation Team on Identification and Excavation of Elite Crop Germplasm, CAAS
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Ultrasonic treatment was performed on water-extractable polysaccharides from the seed of mung beans. Purified by anion-exchange and gel filtration chromatography, MWP-1' and MWP-2' were obtained. Average molecular weights (MwS) of MWP-1' and MWP-2' were 68.4 kDa, and 52.4 kDa, respectively. Monosaccharides components analysis indicated that MWP-1' was composed of Rha, Ara, Man and Gal in a molar percent of 0.4:2.6:5.3:0.7. MWP-2' was composed of Ara, Man, Gal and Glc in a molar percent of 0.5:1.4:2.1:0.4. In vitro study showed that both polysaccharides samples were able to stimulate the production of secretory molecules (NO, TNE-alpha. and IL-6) of RAW264.7 murine macrophages in a dosage dependent manner. MWP-2' seemed to be the most potent and induced significantly higher the NO production. These findings suggest that the ultrasonic treatment polysaccharides isolated in our study have immune potentiation effects on macrophages. (C) 2015 Elsevier Ltd. All rights reserved.
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