Journal
CARBOHYDRATE POLYMERS
Volume 145, Issue -, Pages 71-77Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.03.020
Keywords
Flammulina velutipes residue; Polysaccharide; Purification; Characterization; Antioxidant activity
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Funding
- Special Fund for Agroscientific Research in the Public Interest [201503142]
- China Postdoctoral Science Foundation [2015M582236]
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In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS(+), DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310 Da, respectively. The glycosyl residue of FVRP-1 was an alpha-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were p -type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1
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