Journal
CARBOHYDRATE POLYMERS
Volume 145, Issue -, Pages 1-12Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.03.009
Keywords
Bacterial cellulose; Laccase; Antimicrobial; Wound dressing; Immobilization; Cytotoxicity
Categories
Funding
- Programa Compromisso para a Cienecia, Portugal, FEDER funding on the Programa Operacional Factores de Competitividade-COMPETE [C2011-UMINHO-2C2T-01]
- national funds through FCT-Foundation for Science and Technology within the scope of the project [POCI-01-0145-FEDER-007136, UID/CTM/00264]
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/64901/2009, SFRH/BPD/64958/2009]
- FCT [PEst-OE/EQB/LA0023/2013]
- Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER [NORTE-07-0124-FEDER-000027]
- [RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462)]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/64901/2009, UID/CTM/00264/2013] Funding Source: FCT
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This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I alpha cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol(-1) for 2,6-dimethylphenol (DMP), catechol and 2,2 '-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92 %) and Gram-negative (26 %) bacteria and cytotoxicity acceptable for wound dressing applications. (C) 2016 Elsevier Ltd. All rights reserved.
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