Journal
CARBOHYDRATE POLYMERS
Volume 150, Issue -, Pages 149-158Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.05.011
Keywords
BCG-PSN; Structure; Chain conformation; Immunomodulatory activity
Categories
Funding
- National Scientific and Technological Major Project for Significant New Drugs Creation [2012ZX09502001-004]
- National Natural Science Foundation of China [31301473, 81473216]
- Innovative Scientific Research Team Fund
- Qing Lan Project of Jiangsu Province
- 333 project of Jiangsu Province [BRA2015322]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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A polysaccharide, coded as BDP, purified from the injection powder of Bacillus Calmette Guerin (BCG) polysaccharide and nucleic acid, was composed mainly of alpha-D-(1 -> 4)-linked glucan with (1 -> 6)-linked branches and trace amounts of fucose and mannose from the results of FT-IR, HPAEC-PAD and NMR spectrum. The 4,, Mz, and < s(2)> z(1/2) were determined to be 1.320 x 10(5) g/mol, 1.012 x 10(5) g/mol, 2.139 x 105 g/mol, and 21.8 +/- 3.2%nm by using HPSEC-MALLS, respectively. The v value from (s(2))z(1/2) KMWv was calculated to be 0.52 +/- 0.01, which firstly clarified that BDP existed as random coils in 0.9% NaCl aqueous solution. AFM and SEM combined with Congo -red test also revealed that the polysaccharide was irregular globular like or curly structure. Furthermore, in vitro tests on RAW264.7 murine macrophages cells revealed that BDP exhibited significant immunomodulatory activity. (C) 2016 Elsevier Ltd. All rights reserved.
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