Journal
INSECTS
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/insects11030168
Keywords
cicadidae periostracum; Cryptotympana atrata; cytochrome C oxidase subunit I; molecular authentication; sequence-characterized amplified region
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Funding
- Establishment of Application Base for Chung-bu Medicinal Materials Described in the Dong Ui Bo Gam [KSN1812410]
- Development of Sustainable Application for Standard Herbal Resources from the Korea Institute of Oriental Medicine [KSN2012320]
- National Research Council of Science & Technology (NST), Republic of Korea [KSN2012320] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson's disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People's Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. atrata from the three other cicada species, and detected the adulteration of market CP samples. This SCAR assay is a rapid, simple, cheap, reliable, and reproducible method for species identification, regardless of sample form and status, and contributes to CP quality control.
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