Journal
CARBOHYDRATE POLYMERS
Volume 153, Issue -, Pages 512-517Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.07.122
Keywords
Pyropia yezoensis; Microwave-assisted rapid enzyme digest system; Low molecular weight polysaccharides; Antioxidative effect
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Funding
- Ministry of Agriculture, Food and Rural Affairs (MAFRA)
- Ministry of Oceans and Fisheries (MOF)
- Korea Forest Service (KFS) [213004-04-4-SBA30]
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This study describes a simple preparation of functional polysaccharides from Pyropia yezoensis using a microwave-assistant rapid enzyme digest system (MAREDS) with various carbohydrases, and evaluates their antioxidative effects. Polysaccharide hydrolysates were prepared using MAREDS under different hydrolytic conditions of the carbohydrases and microwave powers. Polysaccharides less than 10 kDa (Low molecular weight polysaccharides, LMWP, <= 10 kDa) were efficiently obtained using an ultrafiltration (molecular weight cut-off of 10 kDa). MAREDS increases AMG activation via an increased degree of hydrolysis; the best AMG hydrolysate was prepared using a 10:1 ratio of substrate to enzyme for 2 h in MAREDS with 400W. LMWP consisted of galactose (27.3%), glucose (64.5%), and mannose (8.3%) from the AMG hydrolysate had stronger antioxidant effects than the high molecular weight polysaccharides (>10 kDa). We rapidly prepared functional LMWPs by using MAREDS with carbohydrases, and suggest that LMWP might be potentially a valuable algal polysaccharide antioxidant. (C) 2016 Elsevier Ltd. All rights reserved.
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