Journal
VIROLOGICA SINICA
Volume 35, Issue 2, Pages 191-199Publisher
KEAI PUBLISHING LTD
DOI: 10.1007/s12250-019-00175-4
Keywords
Pseudorabies virus (PRV); Bacterial artificial chromosome (BAC); Base-editing; CRISPR; Cas9; Genome editing
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Funding
- National Key Research and Development Program [2016YFD0500105]
- Natural Science Foundation of China [31770191]
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Viruses evolve rapidly and continuously threaten animal health and economy, posing a great demand for rapid and efficient genome editing technologies to study virulence mechanism and develop effective vaccine. We present a highly efficient viral genome manipulation method using CRISPR-guided cytidine deaminase. We cloned pseudorabies virus genome into bacterial artificial chromosome, and used CRISPR-guided cytidine deaminase to directly convert cytidine (C) to uridine (U) to induce premature stop mutagenesis in viral genes. The editing efficiencies were 100%. Comprehensive bioinformatic analysis revealed that a large number of editable sites exist in pseudorabies virus (PRV) genomes. Notably, in our study viral genome exists as a plasmid in E. coli, suggesting that this method is virus species-independent. This application of base-editing provided an alternative approach to generate mutant virus and might accelerate study on virulence and vaccine development.
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