Journal
CARBOHYDRATE POLYMERS
Volume 144, Issue -, Pages 305-314Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.02.068
Keywords
Nemacystus decipiens; Fucoidan; Structure; Anti-angiogenic activity; Bone morphogenetic protein 4
Categories
Funding
- New Drug Creation and Manufacturing Program [2012ZX09301001-003]
- key program of National Natural Science Foundation of China [31230022]
- National Natural Science Foundation of China [31400690]
Ask authors/readers for more resources
A sulfated and acetylated fucoidan, named NDH01, was extracted from seaweed Nemacystus decipiens. NDH01 was composed of mannose, glucuronic acid, fucose, sulfate group and acetyl group in the molar ratio of 3.0: 14.4: 82.6: 34.3: 13.9. The backbone of NDH01 was fucose-free core, composed of alpha-D-1,2-Manp and beta-D-1,4-GlcpA disaccharide repeat unit. The branches were attached at the C3, C4 and C6 of alpha-D-1,2-Manp. The sidechain was composed of alpha-L-1,3,4-Fucp, alpha-L-1,4-Fucp, alpha-L-1,3-Fucp and alpha-L-1,4-GlcpA. The sulfate group was linked to C4 of alpha-L-1,3,4-Fucp, whereas, acetyl group was branched on C2 of alpha-L-1,2,3-Fucp. NDH01 could disrupt tube formation and inhibit the migration as well as cell growth of human microvascular endothelial cells. Besides, phosphorylation of Smad/1/5/8, Erk and FAK was significantly inhibited by NDH01. Further studies uncovered that NDH01 blocked Smad1/5/8 signaling via interacting with bone morphogenetic protein 4 and downregulating bone morphogenetic protein 4 expression. The results suggested that NDH01 might be an angiogenesis inhibitor through targeting bone morphogenetic protein 4. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available