Journal
CARBOHYDRATE POLYMERS
Volume 142, Issue -, Pages 230-239Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.01.058
Keywords
Selenium rich exopolysaccharide; Organo-selenium; Biosynthesis; Antioxidant activities; Pseudomonas PT-8
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Funding
- National Natural Science Foundation of China [31370554]
- China Scholarship Council
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Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8. The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium. Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition. Crude Se-EPS was purified into two fractions a pH neutral Se-EPS-1 and an acidic Se-EPS-2. Structure and chemical composition of Se-EPS-2 were investigated by chromatographic analyses. Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively. Compared to the EPS, the content of rhamnose in Se-EPS increased and molecular weight decreased. The Se-EPS had strong scavenging actions on DPPH center dot,center dot OH and center dot O-2(-), which is much higher than the EPS. (C) 2016 Elsevier Ltd. All rights reserved.
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