Journal
CARBOHYDRATE POLYMERS
Volume 144, Issue -, Pages 371-381Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.01.026
Keywords
Self-assembling nanosystems; Hyaluronic acid; Targeted drug delivery; Inflammatory bowel disease; Budesonide
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Funding
- Tehran University of Medical Sciences [92-02-33-23339]
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To develop a nanoparticulate drug carrier for targeting of the inflamed intestinal mucosa, amphiphilic hyaluronic acid (HA) conjugates were synthesized, which could form self-assembled nanoparticles (NPs) in aqueous solution and budesonide (BDS) was loaded into the HANPs. Their particle sizes were in the range of 177 to 293 nm with negative surface charge. The model of inflammatory CACO-2 cells was utilized to investigate the therapeutic potential of budesonide loaded HA nanocarriers. The highest expression of CD44 receptors was found on inflamed Caco-2 cells, as determined by flow cytometry. FITC-labeled HANPs revealed greater uptake in inflamed CACO-2 cells compared to untreated CACO-2 and CD44-negative cell lines, NIH3T3. BDS loaded HANPs displayed almost no toxicity indicating HANPs are excellent biocompatible nano-carriers. BDS loaded HANPs demonstrated higher anti-inflammatory effect on IL-8 and TNF-alpha secretion in inflamed cell model compared to the same dose of free drug. These results revealed the promising potential of HA nanoparticles as a targeted drug delivery system for IBD treatment. (C) 2016 Elsevier Ltd. All rights reserved.
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