Modification of lily polysaccharide by selenylation and the immune-enhancing activity

Lily polysaccharide (LP) was extracted, purified and selenizingly modified by HNO3-Na2SeO3 method according to L-9(3(4)) orthogonal design. Nine selenizing LPs, sLP(1)-sLP(9), were obtained and their immune enhancing activities were compared taking unmodified LP as control. The results in vitro test showed that sLP(6) presented the strongest activity in promoting lymphocytes proliferation in single and synergetic with PHA, and the relative expression level of IL-2, IL-6 and IFN-gamma mRNA of chicken peripheral lymphocytes. The results in vivo test showed that sLP(6) could promote lymphocytes proliferation and enhance the serum antibody titers and serum IL-2, IL-6, IFN-gamma contents more significantly than LP in chickens vaccinated with Newcastle Disease (ND) vaccine. These results indicate that polysaccharide selenizing can significantly enhance the immune-enhancing activity of LP and the optimal modification conditions are 400 mg of Na2SeO3 per 500 mg of LP, the reaction temperature of 70 degrees C and the reaction time of 6 h. (C) 2016 Elsevier Ltd. All rights reserved.