Journal
CARBOHYDRATE POLYMERS
Volume 142, Issue -, Pages 141-148Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.01.021
Keywords
Ganoderma lucidum polysaccharides; Liposome; Immune response; OVA
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Funding
- National Natural Science Foundation of China [31372472]
- Special Fund for Agro-scientific Research in the Public Interest [201303046, 201403051]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Liposome-based vaccine delivery systems are known to enhance immune responses. Ganoderma lucidum polysaccharides (GLP) have been widely studied as immunomodulator and it could be as inducers of strong immune responses. In the research, GLP and ovalbumin (OVA) were encapsulated into liposome as vaccine and inoculated to mice. The magnitude and kinetics of the humoral and cellular immune responses were investigated. The results showed that GLP-OVA-loaded liposomes (GLPL/OVA) could induce more powerful antigen-specific immune responses than each single-component formulation. Mice immunized with GLPL/OVA displayed higher antigen-specific IgG antibodies, better splenocytes proliferation, higher cytokine secretion by splenocytes and significant activation of CD3+CD4+ and CD3+CD8+ T cells. Thus the GLPL/OVA formulation produced a heightened humoral and cellular immune response, with an overall Thl bias. Enhanced immune responses elicited by the GLPL/OVA formulation might be attributed to effective activation and mature of DC in draining lymph nodes. Overall, these findings indicate that GLPL have the potential to enhance immune responses as vaccine delivery systems. (C) 2016 Elsevier Ltd. All rights reserved.
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