Journal
BIOTECHNOLOGY ADVANCES
Volume 33, Issue 1, Pages 41-52Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2014.12.006
Keywords
CRISPR; Cas9; Site-specific nuclease; Genome editing; Targeted mutagenesis; Gene targeting; Plants
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Funding
- European Research Council Advanced Grant 'Future-Pharma' [269110]
- European Research Council (ERC) [269110] Funding Source: European Research Council (ERC)
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Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Here we describe the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a recently developed tool for the introduction of site-specific double-stranded DNA breaks. We highlight the strengths and weaknesses of this technology compared with two well-established genome editing platforms: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). We summarize recent results obtained in plants using CRISPR/Cas9 technology, discuss possible applications in plant breeding and consider potential future developments. (C) 2015 Elsevier Inc.
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