Journal
CARBOHYDRATE POLYMERS
Volume 141, Issue -, Pages 10-19Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.12.037
Keywords
Lactobacillus reuteri; Exopolysaccharides; ETEC; Adherence; Immunomodulation
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Funding
- VEGA [1/0834/12]
- Probiotech [26220220152]
- European Fund [OAE010101]
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The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol (TM) (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were D-glucan polysaccharides with higher molecular weight (M-w), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1 -> 4) and (1 -> 6) glycosidic linkages, increased IL-1 beta gene expression (0.1 mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1 -> 3) and (1 -> 6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-kappa B, TNF-alpha. and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1 beta and IL-6 (P<0.01). (C) 2015 Elsevier Ltd. All rights reserved.
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