Journal
CELLS
Volume 9, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/cells9010141
Keywords
breast cancer; extracellular vesicles; exosomes; microRNA; biomarker; EV characterization
Categories
Funding
- National Breast Cancer Research Institute (NBCRI)
- Irish Research Council [GOIPG/2016/978]
- Irish Cancer Society collaborative cancer research centre BREAST-PREDICT Grant [CCRC13GAL]
- Wellcome Trust Biomedical Vacation Scholarship [202418/Z/16/Z]
- Health Research Board of Ireland [SS-2016-1744, HRA_POR/2013/341]
- Programme for Research in Third Level Institutions (PRTLI)
- EC FP7 Grant MULTIFUN [262943]
- Science Foundation Ireland [SFI/12/RC/2278]
- Irish Research Council (IRC) [GOIPG/2016/978] Funding Source: Irish Research Council (IRC)
- Wellcome Trust [202418/Z/16/Z] Funding Source: Wellcome Trust
- Health Research Board (HRB) [SS-2016-1744, HRA-POR-2013-341] Funding Source: Health Research Board (HRB)
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Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship between EV protein yield and EV quantity determined by NTA, highlighting the requirement for direct quantification. Using AQ-PCR to relate miRNA copy number to EV yield, a significant increase in miRNA-451a copies/EV was detected in BC patient sera, suggesting potential as a novel biomarker of breast cancer.
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