Journal
CELLS
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/cells8121628
Keywords
hematopoiesis; hematopoietic stem cells; stem cell culture; 2D culture; 3D culture
Categories
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) from Brazil
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) from Brazil
- Instituto Nacional de Ciencia e Tecnologia-Fluidos Complexos (INCT-FCx) from Brazil
- Instituto Nacional de Ciencia e Tecnologia-Medicina Regenerativa (INCT-Regenera) from Brazil
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Hematopoiesis is the main function of bone marrow. Human hematopoietic stem and progenitor cells reside in the bone marrow microenvironment, making it a hotspot for the development of hematopoietic diseases. Numerous alterations that correspond to disease progression have been identified in the bone marrow stem cell niche. Complex interactions between the bone marrow microenvironment and hematopoietic stem cells determine the balance between the proliferation, differentiation and homeostasis of the stem cell compartment. Changes in this tightly regulated network can provoke malignant transformation. However, our understanding of human hematopoiesis and the associated niche biology remains limited due to accessibility to human material and the limits of in vitro culture models. Traditional culture systems for human hematopoietic studies lack microenvironment niches, spatial marrow gradients, and dense cellularity, rendering them incapable of effectively translating marrow physiology ex vivo. This review will discuss the importance of 2D and 3D culture as a physiologically relevant system for understanding normal and abnormal hematopoiesis.
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