Journal
CANCERS
Volume 12, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/cancers12010232
Keywords
drug sensitivity; immune infiltration; renal cancer; targeted therapy; tumor slice culture
Categories
Funding
- INSERM
- CEA
- Ligue Nationale contre le Cancer
- Ligue Comite de l'Isere
- University Grenoble Alpes
- Centre Hospitalier Universitaire de Grenoble-Alpes (CHUGA)
- Groupement des Entreprises Francaises dans la LUtte contre le Cancer (GEFLUC)
- Grenoble Alliance for Integrated Structural & Cell Biology (GRAL)
- Association Francaise d'Urologie (AFU)
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Clear cell renal cell carcinoma (ccRCC) is the third type of urologic cancer. At time of diagnosis, 30% of cases are metastatic with no effect of chemotherapy or radiotherapy. Current targeted therapies lead to a high rate of relapse and resistance after a short-term response. Thus, a major hurdle in the development and use of new treatments for ccRCC is the lack of good pre-clinical models that can accurately predict the efficacy of new drugs and allow the stratification of patients into the correct treatment regime. Here, we describe different 3D cultures models of ccRCC, emphasizing the feasibility and the advantage of ex-vivo treatment of fresh, surgically resected human tumor slice cultures of ccRCC as a robust preclinical model for identifying patient response to specific therapeutics. Moreover, this model based on precision-cut tissue slices enables histopathology measurements as tumor architecture is retained, including the spatial relationship between the tumor and tumor-infiltrating lymphocytes and the stromal components. Our data suggest that acute treatment of tumor tissue slices could represent a benchmark of further exploration as a companion diagnostic tool in ccRCC treatment and a model to develop new therapeutic drugs.
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