Journal
JOURNAL OF CLINICAL MEDICINE
Volume 9, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/jcm9010090
Keywords
anoikis; cancer chemoprevention; carotenoid; colorectal cancer; fucoxanthin
Categories
Funding
- Japan Society for the Promotion of Science KAKENHI [16K07880]
- Grants-in-Aid for Scientific Research [16K07880] Funding Source: KAKEN
Ask authors/readers for more resources
Fucoxanthin (Fx), abundantly contained in edible brown algae, is a carotenoid with strong anti-cancer potential. Anoikis is an anchor-dependent apoptosis particularly related to integrin signaling, and a target for cancer preventive strategies. We recently demonstrated that Fx prevented colon cancer in azoxymethane-dextrane sodium sulfate (AOM/DSS) carcinogenic model mice, and that it increased anoikis-like integrin beta 1(low/-)/cleaved caspase-3(high) cells in colonic mucosal crypts. However, an induction mechanism of anoikis by Fx in adenocarcinoma tissue remains unresolved. Thus, we investigated anoikis in colonic adenocarcinoma in AOM/DSS mice. Fx administration (30 mg/kg body weight) significantly suppressed the incidence and multiplicity of colonic adenocarcinoma in AOM/DSS mice. A number of anoikis-like integrin beta 1(low/-)/cleaved caspase-3(high) cells in colonic adenocarcinoma and mucosal crypts were significantly increased, 8.3- and 3.5-fold in the Fx group compared with those of the control group, respectively. The results indicated the increase of anoikis-like cells occurred more strongly in colonic adenocarcinoma than in colonic mucosal crypts. In addition, integrin beta 1 expression, and pFAK (Tyr(397)) and pPaxillin (Tyr(31)) activation in mucosal tissue decreased 0.7-, 0.5- and 0.6-fold by Fx administration, respectively. The results suggest that Fx induces anoikis in colonic adenocarcinoma developed by AOM/DSS treatment through attenuation of integrin signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available