Journal
ACS SENSORS
Volume 5, Issue 3, Pages 807-813Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b02454
Keywords
biosensor; DNA; bioluminescence; PNA; smartphone readout
Funding
- SNSF [200020_169141, 200020_188406]
- NCCR Chemical Biology
- Swiss National Science Foundation (SNF) [200020_169141, 200020_188406] Funding Source: Swiss National Science Foundation (SNF)
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Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.
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